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m, and a rise in the standard quantity of Zn sure to the protein, suggesting that an additional metal ion can bind to this mutant. The drop in catalytic exercise may end result from the binding of this second metal ion or to steric crowding throughout the confines of an Lively web site that has apparently evolved to work with a single divalent cation.
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cat vs. pH-profile demonstrated in Figure 2A for Zn-NagA was match to equation three and these success point out which the deprotonation of one acid by using a kinetic p
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